- Author:
- Shelley Fong <sfon036@UoA.auckland.ac.nz>
- Date:
- 2024-09-06 11:20:56+12:00
- Desc:
- Adding cellml version that imports GPCR_M2
- Permanent Source URI:
- http://models.cellml.org/workspace/bbf/rawfile/0b71dd63f14c188f136239d3e201b3208b2d5a22/BG_KACh_import_M2.cellml
<?xml version='1.0' encoding='UTF-8'?>
<model name="KACh_model" xmlns="http://www.cellml.org/cellml/1.1#" xmlns:cellml="http://www.cellml.org/cellml/1.1#" xmlns:xlink="http://www.w3.org/1999/xlink">
<import xlink:href="units_and_constants/units_BG.cellml">
<units name="mM" units_ref="mM"/>
<units name="per_mol" units_ref="per_mol"/>
<units name="J_per_mol" units_ref="J_per_mol"/>
<units name="J_per_C" units_ref="J_per_C"/>
<units name="J_per_K_per_mol" units_ref="J_per_K_per_mol"/>
<units name="mol_per_s" units_ref="mol_per_s"/>
<units name="C_per_mol" units_ref="C_per_mol"/>
<units name="microm3" units_ref="microm3"/>
<units name="fF" units_ref="fF"/>
<units name="fC" units_ref="fC"/>
<units name="fA" units_ref="fA"/>
<units name="fmol" units_ref="fmol"/>
<units name="fmol_per_sec" units_ref="fmol_per_sec"/>
<units name="per_fmol" units_ref="per_fmol"/>
<units name="pL" units_ref="pL"/>
<units name="mA_per_mM" units_ref="mA_per_mM"/>
</import>
<import xlink:href="GPCR_M2/BG_GPCR_M2_reduced.cellml">
<component component_ref="GPCR_M2_reduced" name="GPCR_M2_reduced"/>
</import>
<import xlink:href="GPCR_M2/BG_GPCR_M2_reduced.cellml">
<component component_ref="GPCR_M2_reduced_parameters" name="GPCR_M2_reduced_parameters"/>
</import>
<import xlink:href="units_and_constants/constants_BG.cellml">
<component component_ref="constants" name="constants"/>
</import>
<component name="environment">
<variable name="t" public_interface="out" units="second"/>
<variable initial_value="1e6" name="C_m" public_interface="out" units="fF"/>
<variable initial_value="25.8" name="w_i" public_interface="out" units="pL"/>
<variable initial_value="3.52" name="w_o" public_interface="out" units="pL"/>
<variable initial_value="-8.5e4" name="q_mem" public_interface="out" units="fC"/>
<variable initial_value="8.31" name="R" public_interface="out" units="J_per_K_per_mol"/>
<variable initial_value="310" name="T" public_interface="out" units="kelvin"/>
<variable initial_value="96500" name="F" public_interface="out" units="C_per_mol"/>
<variable name="v_KACh" public_interface="in" units="fmol_per_sec"/>
<variable initial_value="9.3276" name="q_K_o" public_interface="out" units="fmol"/>
<variable initial_value="0.00456" name="q_K_i" public_interface="out" units="fmol"/>
<variable name="V_mem" public_interface="out" units="J_per_C"/>
<variable name="I_mem_KACh" public_interface="in" units="fA"/>
<math xmlns="http://www.w3.org/1998/Math/MathML">
<apply>
<eq/>
<apply>
<diff/>
<bvar>
<ci>t</ci>
</bvar>
<ci>q_K_i</ci>
</apply>
<apply>
<minus/>
<ci>v_KACh</ci>
</apply>
</apply>
<apply>
<eq/>
<apply>
<diff/>
<bvar>
<ci>t</ci>
</bvar>
<ci>q_K_o</ci>
</apply>
<ci>v_KACh</ci>
</apply>
<apply>
<eq/>
<apply>
<diff/>
<bvar>
<ci>t</ci>
</bvar>
<ci>q_mem</ci>
</apply>
<ci>I_mem_KACh</ci>
</apply>
<apply>
<eq/>
<ci>V_mem</ci>
<apply>
<divide/>
<ci>q_mem</ci>
<ci>C_m</ci>
</apply>
</apply>
</math>
<!-- M2 things-->
<variable initial_value="0.00072162" name="q_RM2_inactive" public_interface="out" units="fmol"/>
<variable initial_value="1e-18" name="q_L_RM2_inactive" public_interface="out" units="fmol"/>
<variable name="q_LM2" public_interface="out" units="fmol"/>
<variable name="q_LM2_stim" units="fmol"/>
<variable initial_value="0" name="q_LM2_ode" units="fmol"/>
<variable initial_value="1e-18" name="q_RM2" public_interface="out" units="fmol"/>
<variable initial_value="0.00836" name="q_Gi" public_interface="out" units="fmol"/>
<variable initial_value="1e-18" name="q_RM2_Gi" public_interface="out" units="fmol"/>
<variable initial_value="1e-18" name="q_L_RM2" public_interface="out" units="fmol"/>
<variable initial_value="1e-18" name="q_L_RM2_Gi" public_interface="out" units="fmol"/>
<variable initial_value="1e-18" name="q_Gia_GTP" public_interface="out" units="fmol"/>
<variable initial_value="1e-18" name="q_Gibetagamma" public_interface="out" units="fmol"/>
<variable initial_value="1e-18" name="q_Gia_GDP" public_interface="out" units="fmol"/>
<variable initial_value="2.2" name="q_GTP" public_interface="out" units="fmol"/>
<variable initial_value="1.1" name="q_GDP" public_interface="out" units="fmol"/>
<variable initial_value="570" name="q_Pi" public_interface="out" units="fmol"/>
<variable initial_value="1e-18" name="q_RM2_tag" public_interface="out" units="fmol"/>
<variable initial_value="1e-18" name="q_L_RM2_tag" public_interface="out" units="fmol"/>
<variable initial_value="1e-18" name="q_RM2_GRKArr" public_interface="out" units="fmol"/>
<variable initial_value="1e-18" name="q_L_RM2_GRKArr" public_interface="out" units="fmol"/>
<variable initial_value="1e-18" name="q_GRKArr" public_interface="out" units="fmol"/>
<!-- stimulus-->
<variable initial_value="7e1" name="stimSt" units="second"/>
<variable initial_value="5e1" name="stimDur" units="second"/>
<variable initial_value="5e1" name="tR" units="second"/>
<variable initial_value="0e2" name="stimMag" units="fmol"/>
<variable initial_value="1e-8" name="stimHolding" units="fmol"/>
<variable name="m" units="fmol_per_sec"/>
<math xmlns="http://www.w3.org/1998/Math/MathML">
<apply>
<eq/>
<ci>m</ci>
<apply>
<divide/>
<ci>stimMag</ci>
<ci>tR</ci>
</apply>
</apply>
<apply>
<eq/>
<ci>q_LM2_stim</ci>
<piecewise>
<piece>
<apply>
<plus/>
<ci>stimHolding</ci>
<apply>
<times/>
<ci>m</ci>
<apply>
<plus/>
<apply>
<minus/>
<ci>t</ci>
<ci>stimSt</ci>
</apply>
<ci>tR</ci>
</apply>
</apply>
</apply>
<apply>
<and/>
<apply>
<lt/>
<ci>t</ci>
<ci>stimSt</ci>
</apply>
<apply>
<gt/>
<ci>t</ci>
<apply>
<minus/>
<ci>stimSt</ci>
<ci>tR</ci>
</apply>
</apply>
</apply>
</piece>
<piece>
<apply>
<plus/>
<ci>stimMag</ci>
<ci>stimHolding</ci>
</apply>
<apply>
<and/>
<apply>
<geq/>
<ci>t</ci>
<ci>stimSt</ci>
</apply>
<apply>
<lt/>
<ci>t</ci>
<apply>
<plus/>
<ci>stimSt</ci>
<ci>stimDur</ci>
</apply>
</apply>
</apply>
</piece>
<piece>
<apply>
<plus/>
<ci>stimHolding</ci>
<apply>
<times/>
<apply>
<minus/>
<ci>m</ci>
</apply>
<apply>
<minus/>
<apply>
<minus/>
<apply>
<minus/>
<ci>t</ci>
<ci>stimSt</ci>
</apply>
<ci>tR</ci>
</apply>
<ci>stimDur</ci>
</apply>
</apply>
</apply>
<apply>
<and/>
<apply>
<lt/>
<ci>t</ci>
<apply>
<plus/>
<ci>stimSt</ci>
<ci>tR</ci>
<ci>stimDur</ci>
</apply>
</apply>
<apply>
<geq/>
<ci>t</ci>
<apply>
<plus/>
<ci>stimSt</ci>
<ci>stimDur</ci>
</apply>
</apply>
</apply>
</piece>
<otherwise>
<ci>stimHolding</ci>
</otherwise>
</piecewise>
</apply>
</math>
<!-- mass conservation checks-->
<variable name="conserv_R_T" units="fmol"/>
<variable name="conserv_L_T" units="fmol"/>
<variable name="conserv_G_T" units="fmol"/>
<variable name="total_R" units="fmol"/>
<math xmlns="http://www.w3.org/1998/Math/MathML">
<apply>
<eq/>
<ci>conserv_R_T</ci>
<apply>
<plus/>
<ci>q_RM2_inactive</ci>
<ci>q_L_RM2_inactive</ci>
<ci>q_RM2</ci>
<ci>q_RM2_Gi</ci>
<ci>q_L_RM2</ci>
<ci>q_L_RM2_Gi</ci>
<ci>q_RM2_tag</ci>
<ci>q_L_RM2_tag</ci>
<ci>q_RM2_GRKArr</ci>
<ci>q_L_RM2_GRKArr</ci>
</apply>
</apply>
<apply>
<eq/>
<ci>total_R</ci>
<apply>
<plus/>
<ci>q_RM2_inactive</ci>
<ci>q_L_RM2_inactive</ci>
<ci>q_RM2</ci>
<ci>q_RM2_Gi</ci>
<ci>q_L_RM2</ci>
<ci>q_L_RM2_Gi</ci>
</apply>
</apply>
<apply>
<eq/>
<ci>conserv_L_T</ci>
<apply>
<plus/>
<ci>q_L_RM2_inactive</ci>
<ci>q_LM2</ci>
<ci>q_L_RM2</ci>
<ci>q_L_RM2_Gi</ci>
<ci>q_L_RM2_tag</ci>
<ci>q_L_RM2_GRKArr</ci>
</apply>
</apply>
<apply>
<eq/>
<ci>conserv_G_T</ci>
<apply>
<plus/>
<ci>q_Gi</ci>
<ci>q_RM2_Gi</ci>
<ci>q_L_RM2_Gi</ci>
<ci>q_Gia_GTP</ci>
<ci>q_Gia_GDP</ci>
</apply>
</apply>
</math>
<!-- From submodule-->
<variable name="v_Rswitch_M2" public_interface="in" units="fmol_per_sec"/>
<variable name="v_LRswitch_M2" public_interface="in" units="fmol_per_sec"/>
<variable name="v_C_M2" public_interface="in" units="fmol_per_sec"/>
<variable name="v_R_M2" public_interface="in" units="fmol_per_sec"/>
<variable name="v_L_M2" public_interface="in" units="fmol_per_sec"/>
<variable name="v_Act1_Gi" public_interface="in" units="fmol_per_sec"/>
<variable name="v_Act2_Gi" public_interface="in" units="fmol_per_sec"/>
<variable name="v_Hyd_Gi" public_interface="in" units="fmol_per_sec"/>
<variable name="v_Reassoc_Gi" public_interface="in" units="fmol_per_sec"/>
<variable name="v_InternR_M2" public_interface="in" units="fmol_per_sec"/>
<variable name="v_InternLR_M2" public_interface="in" units="fmol_per_sec"/>
<variable name="v_Rsynthesis" units="fmol_per_sec"/>
<math xmlns="http://www.w3.org/1998/Math/MathML">
<apply>
<eq/>
<ci>v_Rsynthesis</ci>
<apply>
<plus/>
<apply>
<plus/>
<ci>v_C_M2</ci>
</apply>
<ci>v_L_M2</ci>
</apply>
</apply>
<apply>
<eq/>
<apply>
<diff/>
<bvar>
<ci>t</ci>
</bvar>
<ci>q_RM2_inactive</ci>
</apply>
<apply>
<minus/>
<apply>
<minus/>
<ci>v_Rswitch_M2</ci>
</apply>
<ci>v_L_M2</ci>
</apply>
</apply>
<apply>
<eq/>
<apply>
<diff/>
<bvar>
<ci>t</ci>
</bvar>
<ci>q_L_RM2_inactive</ci>
</apply>
<apply>
<minus/>
<ci>v_LRswitch_M2</ci>
</apply>
</apply>
<apply>
<eq/>
<apply>
<diff/>
<bvar>
<ci>t</ci>
</bvar>
<ci>q_LM2_ode</ci>
</apply>
<apply>
<minus/>
<ci>v_L_M2</ci>
</apply>
</apply>
<apply>
<eq/>
<apply>
<diff/>
<bvar>
<ci>t</ci>
</bvar>
<ci>q_RM2</ci>
</apply>
<apply>
<plus/>
<apply>
<minus/>
<ci>v_Rswitch_M2</ci>
<ci>v_C_M2</ci>
</apply>
<apply>
<times/>
<cn cellml:units="dimensionless">1.0</cn>
<ci>v_Rsynthesis</ci>
</apply>
</apply>
</apply>
<apply>
<eq/>
<apply>
<diff/>
<bvar>
<ci>t</ci>
</bvar>
<ci>q_Gi</ci>
</apply>
<apply>
<plus/>
<apply>
<minus/>
<apply>
<minus/>
<ci>v_C_M2</ci>
</apply>
<ci>v_R_M2</ci>
</apply>
<ci>v_Reassoc_Gi</ci>
</apply>
</apply>
<apply>
<eq/>
<apply>
<diff/>
<bvar>
<ci>t</ci>
</bvar>
<ci>q_RM2_Gi</ci>
</apply>
<apply>
<minus/>
<ci>v_C_M2</ci>
<ci>v_Act1_Gi</ci>
</apply>
</apply>
<apply>
<eq/>
<apply>
<diff/>
<bvar>
<ci>t</ci>
</bvar>
<ci>q_L_RM2</ci>
</apply>
<apply>
<plus/>
<apply>
<minus/>
<ci>v_LRswitch_M2</ci>
<ci>v_R_M2</ci>
</apply>
<ci>v_L_M2</ci>
</apply>
</apply>
<apply>
<eq/>
<apply>
<diff/>
<bvar>
<ci>t</ci>
</bvar>
<ci>q_L_RM2_Gi</ci>
</apply>
<apply>
<minus/>
<ci>v_R_M2</ci>
<ci>v_Act2_Gi</ci>
</apply>
</apply>
<apply>
<eq/>
<apply>
<diff/>
<bvar>
<ci>t</ci>
</bvar>
<ci>q_Gia_GTP</ci>
</apply>
<apply>
<minus/>
<apply>
<plus/>
<ci>v_Act1_Gi</ci>
<ci>v_Act2_Gi</ci>
</apply>
<ci>v_Hyd_Gi</ci>
</apply>
</apply>
<apply>
<eq/>
<apply>
<diff/>
<bvar>
<ci>t</ci>
</bvar>
<ci>q_Gibetagamma</ci>
</apply>
<apply>
<minus/>
<apply>
<plus/>
<ci>v_Act1_Gi</ci>
<ci>v_Act2_Gi</ci>
</apply>
<ci>v_Reassoc_Gi</ci>
</apply>
</apply>
<apply>
<eq/>
<apply>
<diff/>
<bvar>
<ci>t</ci>
</bvar>
<ci>q_Gia_GDP</ci>
</apply>
<apply>
<minus/>
<ci>v_Hyd_Gi</ci>
<ci>v_Reassoc_Gi</ci>
</apply>
</apply>
<apply>
<eq/>
<apply>
<diff/>
<bvar>
<ci>t</ci>
</bvar>
<ci>q_GTP</ci>
</apply>
<apply>
<minus/>
<apply>
<minus/>
<ci>v_Act1_Gi</ci>
</apply>
<ci>v_Act2_Gi</ci>
</apply>
</apply>
<apply>
<eq/>
<apply>
<diff/>
<bvar>
<ci>t</ci>
</bvar>
<ci>q_GDP</ci>
</apply>
<apply>
<plus/>
<ci>v_Act1_Gi</ci>
<ci>v_Act2_Gi</ci>
</apply>
</apply>
<apply>
<eq/>
<apply>
<diff/>
<bvar>
<ci>t</ci>
</bvar>
<ci>q_Pi</ci>
</apply>
<ci>v_Hyd_Gi</ci>
</apply>
<apply>
<eq/>
<apply>
<diff/>
<bvar>
<ci>t</ci>
</bvar>
<ci>q_RM2_tag</ci>
</apply>
<apply>
<minus/>
<ci>v_Act1_Gi</ci>
<ci>v_InternR_M2</ci>
</apply>
</apply>
<apply>
<eq/>
<apply>
<diff/>
<bvar>
<ci>t</ci>
</bvar>
<ci>q_L_RM2_tag</ci>
</apply>
<apply>
<minus/>
<ci>v_Act2_Gi</ci>
<ci>v_InternLR_M2</ci>
</apply>
</apply>
<apply>
<eq/>
<apply>
<diff/>
<bvar>
<ci>t</ci>
</bvar>
<ci>q_RM2_GRKArr</ci>
</apply>
<ci>v_InternR_M2</ci>
</apply>
<apply>
<eq/>
<apply>
<diff/>
<bvar>
<ci>t</ci>
</bvar>
<ci>q_L_RM2_GRKArr</ci>
</apply>
<ci>v_InternLR_M2</ci>
</apply>
<apply>
<eq/>
<apply>
<diff/>
<bvar>
<ci>t</ci>
</bvar>
<ci>q_GRKArr</ci>
</apply>
<apply>
<minus/>
<apply>
<minus/>
<ci>v_InternR_M2</ci>
</apply>
<ci>v_InternLR_M2</ci>
</apply>
</apply>
<apply>
<eq/>
<ci>q_LM2</ci>
<apply>
<plus/>
<ci>q_LM2_stim</ci>
<ci>q_LM2_ode</ci>
</apply>
</apply>
</math>
</component>
<component name="KACh_parameters">
<variable initial_value="0.217237" name="kappa_KACh" public_interface="out" units="fmol_per_sec"/>
<variable initial_value="0.013549" name="K_K_i" public_interface="out" units="per_fmol"/>
<variable initial_value="0.0899434" name="K_K_o" public_interface="out" units="per_fmol"/>
<variable initial_value="1e-6" name="K_Gibetagamma" public_interface="out" units="per_fmol"/>
<variable initial_value="1" name="zK" public_interface="out" units="dimensionless"/>
</component>
<component name="KACh">
<variable name="t" public_interface="in" units="second"/>
<variable name="R" public_interface="in" units="J_per_K_per_mol"/>
<variable name="T" public_interface="in" units="kelvin"/>
<variable name="zK" public_interface="in" units="dimensionless"/>
<variable name="F" public_interface="in" units="C_per_mol"/>
<variable name="K_K_i" public_interface="in" units="per_fmol"/>
<variable name="K_K_o" public_interface="in" units="per_fmol"/>
<variable name="K_Gibetagamma" public_interface="in" units="per_fmol"/>
<variable name="kappa_KACh" public_interface="in" units="fmol_per_sec"/>
<variable name="q_K_i" public_interface="in" units="fmol"/>
<variable name="q_K_o" public_interface="in" units="fmol"/>
<variable name="q_Gibetagamma" public_interface="in" units="fmol"/>
<variable name="mu_K_i" units="J_per_mol"/>
<variable name="mu_K_o" units="J_per_mol"/>
<variable name="mu_Gibetagamma" units="J_per_mol"/>
<variable name="Af_KACh" units="J_per_mol"/>
<variable name="Ar_KACh" units="J_per_mol"/>
<variable name="Am_KACh" units="J_per_mol"/>
<variable name="q_mem" public_interface="in" units="fC"/>
<variable name="v_KACh" public_interface="out" units="fmol_per_sec"/>
<variable name="I_mem_KACh" public_interface="out" units="fA"/>
<variable name="V_mem" public_interface="in" units="J_per_C"/>
<math xmlns="http://www.w3.org/1998/Math/MathML">
<apply>
<eq/>
<ci>mu_K_i</ci>
<apply>
<times/>
<ci>R</ci>
<ci>T</ci>
<apply>
<ln/>
<apply>
<times/>
<ci>K_K_i</ci>
<ci>q_K_i</ci>
</apply>
</apply>
</apply>
</apply>
<apply>
<eq/>
<ci>mu_K_o</ci>
<apply>
<times/>
<ci>R</ci>
<ci>T</ci>
<apply>
<ln/>
<apply>
<times/>
<ci>K_K_o</ci>
<ci>q_K_o</ci>
</apply>
</apply>
</apply>
</apply>
<apply>
<eq/>
<ci>mu_Gibetagamma</ci>
<apply>
<times/>
<ci>R</ci>
<ci>T</ci>
<apply>
<ln/>
<apply>
<times/>
<ci>K_Gibetagamma</ci>
<ci>q_Gibetagamma</ci>
</apply>
</apply>
</apply>
</apply>
<apply>
<eq/>
<ci>Am_KACh</ci>
<apply>
<times/>
<ci>zK</ci>
<ci>F</ci>
<ci>V_mem</ci>
</apply>
</apply>
<apply>
<eq/>
<ci>Af_KACh</ci>
<apply>
<plus/>
<ci>mu_K_i</ci>
<apply>
<times/>
<ci>zK</ci>
<ci>F</ci>
<ci>V_mem</ci>
</apply>
<ci>mu_Gibetagamma</ci>
</apply>
</apply>
<apply>
<eq/>
<ci>Ar_KACh</ci>
<ci>mu_K_o</ci>
</apply>
<apply>
<eq/>
<ci>v_KACh</ci>
<piecewise>
<piece>
<apply>
<times/>
<ci>kappa_KACh</ci>
<apply>
<minus/>
<apply>
<exp/>
<apply>
<divide/>
<ci>Af_KACh</ci>
<apply>
<times/>
<ci>R</ci>
<ci>T</ci>
</apply>
</apply>
</apply>
<apply>
<exp/>
<apply>
<divide/>
<ci>Ar_KACh</ci>
<apply>
<times/>
<ci>R</ci>
<ci>T</ci>
</apply>
</apply>
</apply>
</apply>
</apply>
<apply>
<eq/>
<ci>Am_KACh</ci>
<cn cellml:units="J_per_mol">0</cn>
</apply>
</piece>
<otherwise>
<apply>
<times/>
<apply>
<divide/>
<apply>
<divide/>
<apply>
<times/>
<ci>kappa_KACh</ci>
<ci>Am_KACh</ci>
</apply>
<apply>
<times/>
<ci>R</ci>
<ci>T</ci>
</apply>
</apply>
<apply>
<minus/>
<apply>
<exp/>
<apply>
<divide/>
<ci>Am_KACh</ci>
<apply>
<times/>
<ci>R</ci>
<ci>T</ci>
</apply>
</apply>
</apply>
<cn cellml:units="dimensionless">1</cn>
</apply>
</apply>
<apply>
<minus/>
<apply>
<exp/>
<apply>
<divide/>
<ci>Af_KACh</ci>
<apply>
<times/>
<ci>R</ci>
<ci>T</ci>
</apply>
</apply>
</apply>
<apply>
<exp/>
<apply>
<divide/>
<ci>Ar_KACh</ci>
<apply>
<times/>
<ci>R</ci>
<ci>T</ci>
</apply>
</apply>
</apply>
</apply>
</apply>
</otherwise>
</piecewise>
</apply>
<apply>
<eq/>
<ci>I_mem_KACh</ci>
<apply>
<times/>
<apply>
<minus/>
<ci>zK</ci>
</apply>
<ci>F</ci>
<ci>v_KACh</ci>
</apply>
</apply>
</math>
</component>
<connection>
<map_components component_1="environment" component_2="KACh"/>
<map_variables variable_1="t" variable_2="t"/>
<map_variables variable_1="R" variable_2="R"/>
<map_variables variable_1="T" variable_2="T"/>
<map_variables variable_1="F" variable_2="F"/>
<map_variables variable_1="v_KACh" variable_2="v_KACh"/>
<map_variables variable_1="I_mem_KACh" variable_2="I_mem_KACh"/>
<map_variables variable_1="q_K_i" variable_2="q_K_i"/>
<map_variables variable_1="q_K_o" variable_2="q_K_o"/>
<map_variables variable_1="q_Gibetagamma" variable_2="q_Gibetagamma"/>
<map_variables variable_1="q_mem" variable_2="q_mem"/>
<map_variables variable_1="V_mem" variable_2="V_mem"/>
</connection>
<connection>
<map_components component_1="KACh" component_2="KACh_parameters"/>
<map_variables variable_1="K_K_i" variable_2="K_K_i"/>
<map_variables variable_1="K_K_o" variable_2="K_K_o"/>
<map_variables variable_1="K_Gibetagamma" variable_2="K_Gibetagamma"/>
<map_variables variable_1="kappa_KACh" variable_2="kappa_KACh"/>
<map_variables variable_1="zK" variable_2="zK"/>
</connection>
<connection>
<map_components component_1="environment" component_2="GPCR_M2_reduced"/>
<map_variables variable_1="t" variable_2="time"/>
<map_variables variable_1="q_RM2_inactive" variable_2="q_RM2_inactive"/>
<map_variables variable_1="q_L_RM2_inactive" variable_2="q_L_RM2_inactive"/>
<map_variables variable_1="q_LM2" variable_2="q_LM2"/>
<map_variables variable_1="q_RM2" variable_2="q_RM2"/>
<map_variables variable_1="q_Gi" variable_2="q_Gi"/>
<map_variables variable_1="q_RM2_Gi" variable_2="q_RM2_Gi"/>
<map_variables variable_1="q_L_RM2" variable_2="q_L_RM2"/>
<map_variables variable_1="q_L_RM2_Gi" variable_2="q_L_RM2_Gi"/>
<map_variables variable_1="q_Gia_GTP" variable_2="q_Gia_GTP"/>
<map_variables variable_1="q_Gibetagamma" variable_2="q_Gibetagamma"/>
<map_variables variable_1="q_Gia_GDP" variable_2="q_Gia_GDP"/>
<map_variables variable_1="q_GTP" variable_2="q_GTP"/>
<map_variables variable_1="q_GDP" variable_2="q_GDP"/>
<map_variables variable_1="q_Pi" variable_2="q_Pi"/>
<map_variables variable_1="q_RM2_tag" variable_2="q_RM2_tag"/>
<map_variables variable_1="q_L_RM2_tag" variable_2="q_L_RM2_tag"/>
<map_variables variable_1="q_RM2_GRKArr" variable_2="q_RM2_GRKArr"/>
<map_variables variable_1="q_L_RM2_GRKArr" variable_2="q_L_RM2_GRKArr"/>
<map_variables variable_1="q_GRKArr" variable_2="q_GRKArr"/>
<map_variables variable_1="v_Rswitch_M2" variable_2="v_Rswitch_M2"/>
<map_variables variable_1="v_LRswitch_M2" variable_2="v_LRswitch_M2"/>
<map_variables variable_1="v_C_M2" variable_2="v_C_M2"/>
<map_variables variable_1="v_R_M2" variable_2="v_R_M2"/>
<map_variables variable_1="v_L_M2" variable_2="v_L_M2"/>
<map_variables variable_1="v_Act1_Gi" variable_2="v_Act1_Gi"/>
<map_variables variable_1="v_Act2_Gi" variable_2="v_Act2_Gi"/>
<map_variables variable_1="v_Hyd_Gi" variable_2="v_Hyd_Gi"/>
<map_variables variable_1="v_Reassoc_Gi" variable_2="v_Reassoc_Gi"/>
<map_variables variable_1="v_InternR_M2" variable_2="v_InternR_M2"/>
<map_variables variable_1="v_InternLR_M2" variable_2="v_InternLR_M2"/>
</connection>
<connection>
<map_components component_1="GPCR_M2_reduced" component_2="GPCR_M2_reduced_parameters"/>
<map_variables variable_1="kappa_Rswitch_M2" variable_2="kappa_Rswitch_M2"/>
<map_variables variable_1="kappa_LRswitch_M2" variable_2="kappa_LRswitch_M2"/>
<map_variables variable_1="kappa_C_M2" variable_2="kappa_C_M2"/>
<map_variables variable_1="kappa_R_M2" variable_2="kappa_R_M2"/>
<map_variables variable_1="kappa_L_M2" variable_2="kappa_L_M2"/>
<map_variables variable_1="kappa_Act1_Gi" variable_2="kappa_Act1_Gi"/>
<map_variables variable_1="kappa_Act2_Gi" variable_2="kappa_Act2_Gi"/>
<map_variables variable_1="kappa_Hyd_Gi" variable_2="kappa_Hyd_Gi"/>
<map_variables variable_1="kappa_Reassoc_Gi" variable_2="kappa_Reassoc_Gi"/>
<map_variables variable_1="kappa_InternR_M2" variable_2="kappa_InternR_M2"/>
<map_variables variable_1="kappa_InternLR_M2" variable_2="kappa_InternLR_M2"/>
<map_variables variable_1="K_RM2_inactive" variable_2="K_RM2_inactive"/>
<map_variables variable_1="K_L_RM2_inactive" variable_2="K_L_RM2_inactive"/>
<map_variables variable_1="K_LM2" variable_2="K_LM2"/>
<map_variables variable_1="K_RM2" variable_2="K_RM2"/>
<map_variables variable_1="K_Gi" variable_2="K_Gi"/>
<map_variables variable_1="K_RM2_Gi" variable_2="K_RM2_Gi"/>
<map_variables variable_1="K_L_RM2" variable_2="K_L_RM2"/>
<map_variables variable_1="K_L_RM2_Gi" variable_2="K_L_RM2_Gi"/>
<map_variables variable_1="K_Gia_GTP" variable_2="K_Gia_GTP"/>
<map_variables variable_1="K_Gibetagamma" variable_2="K_Gibetagamma"/>
<map_variables variable_1="K_Gia_GDP" variable_2="K_Gia_GDP"/>
<map_variables variable_1="K_GTP" variable_2="K_GTP"/>
<map_variables variable_1="K_GDP" variable_2="K_GDP"/>
<map_variables variable_1="K_Pi" variable_2="K_Pi"/>
<map_variables variable_1="K_RM2_tag" variable_2="K_RM2_tag"/>
<map_variables variable_1="K_L_RM2_tag" variable_2="K_L_RM2_tag"/>
<map_variables variable_1="K_RM2_GRKArr" variable_2="K_RM2_GRKArr"/>
<map_variables variable_1="K_L_RM2_GRKArr" variable_2="K_L_RM2_GRKArr"/>
<map_variables variable_1="K_GRKArr" variable_2="K_GRKArr"/>
</connection>
<connection>
<map_components component_1="environment" component_2="GPCR_M2_reduced"/>
<map_variables variable_1="t" variable_2="time"/>
<map_variables variable_1="q_RM2_inactive" variable_2="q_RM2_inactive"/>
<map_variables variable_1="q_L_RM2_inactive" variable_2="q_L_RM2_inactive"/>
<map_variables variable_1="q_LM2" variable_2="q_LM2"/>
<map_variables variable_1="q_RM2" variable_2="q_RM2"/>
<map_variables variable_1="q_Gi" variable_2="q_Gi"/>
<map_variables variable_1="q_RM2_Gi" variable_2="q_RM2_Gi"/>
<map_variables variable_1="q_L_RM2" variable_2="q_L_RM2"/>
<map_variables variable_1="q_L_RM2_Gi" variable_2="q_L_RM2_Gi"/>
<map_variables variable_1="q_Gia_GTP" variable_2="q_Gia_GTP"/>
<map_variables variable_1="q_Gibetagamma" variable_2="q_Gibetagamma"/>
<map_variables variable_1="q_Gia_GDP" variable_2="q_Gia_GDP"/>
<map_variables variable_1="q_GTP" variable_2="q_GTP"/>
<map_variables variable_1="q_GDP" variable_2="q_GDP"/>
<map_variables variable_1="q_Pi" variable_2="q_Pi"/>
<map_variables variable_1="q_RM2_tag" variable_2="q_RM2_tag"/>
<map_variables variable_1="q_L_RM2_tag" variable_2="q_L_RM2_tag"/>
<map_variables variable_1="q_RM2_GRKArr" variable_2="q_RM2_GRKArr"/>
<map_variables variable_1="q_L_RM2_GRKArr" variable_2="q_L_RM2_GRKArr"/>
<map_variables variable_1="q_GRKArr" variable_2="q_GRKArr"/>
<map_variables variable_1="v_Rswitch_M2" variable_2="v_Rswitch_M2"/>
<map_variables variable_1="v_LRswitch_M2" variable_2="v_LRswitch_M2"/>
<map_variables variable_1="v_C_M2" variable_2="v_C_M2"/>
<map_variables variable_1="v_R_M2" variable_2="v_R_M2"/>
<map_variables variable_1="v_L_M2" variable_2="v_L_M2"/>
<map_variables variable_1="v_Act1_Gi" variable_2="v_Act1_Gi"/>
<map_variables variable_1="v_Act2_Gi" variable_2="v_Act2_Gi"/>
<map_variables variable_1="v_Hyd_Gi" variable_2="v_Hyd_Gi"/>
<map_variables variable_1="v_Reassoc_Gi" variable_2="v_Reassoc_Gi"/>
<map_variables variable_1="v_InternR_M2" variable_2="v_InternR_M2"/>
<map_variables variable_1="v_InternLR_M2" variable_2="v_InternLR_M2"/>
</connection>
<connection>
<map_components component_1="constants" component_2="GPCR_M2_reduced"/>
<map_variables variable_1="R" variable_2="R"/>
<map_variables variable_1="T" variable_2="T"/>
</connection>
</model>
