Electrophysiological Modeling of Fibroblasts and Their Interaction with Myocytes
Catherine
Lloyd
Bioengineering Institute, University of Auckland
Model Status
This CellML version of the model runs in PCEnv, COR and JSim to recreate the results in the Figure 7 of the publication. The CVode integrator needs to be used in Jsim to run this model. The units have been checked and are consistent, and we would like to thank Frank Sachse, the original model author, for his advice and assistance in getting the CellML version of this model working. This CellML model represents the coupled fibroblast-myocyte model, 1:1 ratio. This ratio can be changed by altering the value of the variable 'number_of_fibroblasts' in the component 'I_inter'.
Model Structure
Fibroblasts are the most abundant cell type present in the heart. They are arranged in the myocardium, as a three-dimensional network, where they are responsible for the secretion of extracellular matrix proteins, and also play a role in cardiac electrophysiology. Although fibroblasts themselves are electrically in-excitable, they can contribute to the electrophysiology of neighbouring myocytes through fibroblast-myocyte coupling. Although the electrical coupling of fibroblasts and myocytes has been demonstrated experimentally both in vivo and in vitro, the precise role of fibroblasts as electrical bridges between myocytes still remains to be defined.
In the paper described here, Frank Sachse, Alonso Moreno and J. A. Abildskov address this issue by presenting mathematical models of cellular and multi-cellular electrophysiology. A novel mathematical model of cardiac fibroblasts was developed on the basis of experimental data from whole cell patch clamp and polymerase chain reaction studies. This model was then applied, together with models of ventricular myocytes (which were based on the Pandit et al. 2001 model of the rat left ventricular myocyte), to determine the electrophysiological effects of heterogeneous electrical coupling of cells.
model diagram
Schematic diagram of the fibroblast cell model. A Markovian model for the time and voltage dependent outward current (ISHkr) is embedded within an electrophysiological cell model. The ion channel has six different states; five closed states (C0-4) and one open state (O)
The original paper reference is cited below:
Electrophysiological Modeling of Fibroblasts and Their Interaction with Myocytes, F.B. Sachse, A.P. Moreno, and J.A. Abildskov, 2008, JanAnnals of Biomedical Engineering
PubMed ID: 17999190
cardiac
cardiac electrophysiology
electrophysiology
myocyte
fibroblast
2008-07-01T15:36:12+12:00
keyword
Electrophysiological Modeling of Fibroblasts and Their Interaction with Myocytes (Coupled Fibroblast-Myocyte Model)
The University of Auckland, Bioengineering Institute
F
Sachse
B
Added cmeta:id's to allow referencing by PCEnv session
James
Lawson
Richard
James
Lawson
Richard
Sachse, Moreno and Abildskov's 2007 mathematical model of the electrophysiology of fibroblasts and how they interact with myocytes.
Dimensional inconsistencies have been fixed.
Variables V_myo, V_JSR, V_SS, V_NSR (component intracellular_ion_concentrations) units changed from picolitres to microlitres, variables changed to match those in Version 08 of Pandit 2001 model.
RHS multiplied by scaling factor of 1e6 with units nanoamps/milliamps in equation defining variable I_inter_fibro in component 'I_inter' in order to address inconsistencies in unit handling between JSim vs PCEnv and COR
Model now runs to give the same results in PCEnv, COR and Jsim
- version 01 only runs to give correct results in Jsim.
A
Moreno
P
Dimensional inconsistencies have been fixed.
Variables V_myo, V_JSR, V_SS, V_NSR (component intracellular_ion_concentrations) units changed from picolitres to microlitres, variables changed to match those in Version 08 of Pandit 2001 model.
RHS multiplied by scaling factor of 1e6 with units nanoamps/milliamps in equation defining variable I_inter_fibro in component 'I_inter'
Model now runs to give the same results in PCEnv, COR and Jsim
- version 01 only runs to give correct results in Jsm.
James
Lawson
Richard
The University of Auckland
The Bioengineering Institute
This is a CellML description of Sachse, Moreno and Abildskov's 2007 mathematical model of the electrophysiology of fibroblasts and how they interact with myocytes.
2008-01-00 00:00
This CellML version of the model runs in PCEnv, COR and JSim to recreate the results in the published paper. The units have been checked and are consistent, and we would like to thank Frank Sachse, the original model author, for his advice and assistance in getting the CellML version of this model working. This particular CellML model represents the coupled fibroblast-myocyte model.
James
Lawson
Richard
0.12
10000
0.001
Electrophysiological Modeling of Fibroblasts and Their Interaction with Myocytes
36(1)
41
56
2008-06-12T14:45:01+12:00
James
Lawson
Richard
Catherine
Lloyd
May
J
Abildskov
A
Annals of Biomedical Engineering
Catherine Lloyd
2008-06-05T15:15:40+12:00
2007-11-16T00:00:00+00:00
c.lloyd@auckland.ac.nz
Changed simulation metadata to simulate for 0.12 seconds, ala fig7a of publication, added some cmeta:id's to allow referencing of variables by PCEnv sessions.
2008-05-30T14:23:08+12:00
added cmeta id's to allow PCEnv session expansion
2008-05-30T14:23:08+12:00
17999190
Catherine Lloyd