Wolf, Sohn, Heinrich, Kuriyama, 2001

Model Status

This model runs to reproduce published results in COR, OpenCell and PCEnv. The units are consistent throughout. This model currently reproduces Figure 2B, changing KH (to infinity) and f2 (to 1) will allow the model to reproduce Figure 2A. The initial conditions were estimated and the model run to steady state to yield precise values, changing any parameters will necessitate repeating this procedure.

Model Structure

ABSTRACT: Autonomous metabolic oscillations were observed in aerobic continuous culture of Saccharomyces cerevisiae. Experimental investigation of the underlying mechanism revealed that several pathways and regulatory couplings are involved. Here a hypothetical mechanism including the sulfate assimilation pathway, ethanol degradation and respiration is transformed into a mathematical model. Simulations confirm the ability of the model to produce limit cycle oscillations which reproduce most of the characteristic features of the system

The original paper reference is cited below:

Mathematical analysis of a mechanism for autonomous metabolic oscillations in continuous culture of Saccharomyces cerevisiae, Jana Wolf, Ho-Yong Sohn, Reinhart Heinrich and Hiroshi Kuriyama, 2001, FEBS Letters, 499, 230-234. PubMed ID: 11423122

Reaction scheme of the model. The following abbreviations are introduced for the metabolites: sul: sulphate ions, aps: adenylyl sulfate, pap: 3-phosphoadenylyl sulfate, hyd: hydrogen sulphide, cys: cysteine, eth: ethanol, aco: acetyl-CoA, S1 and S2: intermediates of the citric acid cycle, oxy: oxygen, C1 and C2: protein complexes involved in oxidative phosphorylation, A3: ATP, A2: ADP, N1: NAD(P)+, N2: NAD(P)H, oah: O-acetylhomoserine. The cytosolic and mitochondrial compartments are characterized by the superscripts c and m, respectively.