Voltage clamp experiments in striated muscle fibres

Voltage clamp experiments in striated muscle fibres

Model Status

This model has had a stimulus protocol added to it to allow simulation of action potentials. Unfortunately, the details of the stimulation defined in the original paper are not known and as such, the parameters of the stimulus (magnitude, duration, dimensions,) may not be appropriate. Currently, however, the model runs in PCEnv and is able to simulate a train of action potentials. COR will not currently run this model.

ValidateCellML detects unit inconsistencies within this model.

Model Structure

In this 1970 publication, Adrian, Chandler and Hodgkin developed a mathematical model of an action potential in frog striated muscle. Their model was based on data from voltage-clamp experiments, and they include descriptions of three currents:

  • INa , a fast inward sodium current;

  • IK , a slow outward potassium current; and

  • IL , a leak current.

The style of the model equations is based on the The Hodgkin-Huxley Squid Axon Model, 1952. However, the authors acknowledge that the situation in striated muscle is complicated by tubular resistance and capacity. The transverse tubular system which exists in striated myocytes is represented in the model by a linear resistance and capacity in series.

A schematic cell diagram describing the current flows across the cell membrane that are captured in the Hodgkin Huxley model.

As the paper was published in 1970, there is no online version. However, the complete reference is cited below:

Voltage Clamp Experiments in Striated Muscle Fibres. R. H. Adrian, W. K. Chandler, and A. L. Hodgkin. Journal of Physiology, (1970), 208, pp 607-644. PubMed ID: 5499787

In order to complete the model and run simulations, some parameters were also taken from the following paper:

Reconstruction of the Action Potential of Frog Sartorius Muscle. R. H. Adrian and L. D. Peachey. Journal of Physiology, (1973), 235, pp 103-131. PubMed ID: 4778131