Single_stim_experiment.cellml

Single stimulation experiment

In the Single stimulation experiment, the Excitation-contraction component is configured and parameterised with an applied Single pulse patch clamp protocol.

You can change the parameters of stimulation in the component Vstim_para.

You can also bypass the parameters in the component model_parameters and initial_conditions and define your own parameters. We defined control_para to alter some parameters such as intracellular Na⁺ concentration for specific experiments.

The simulation experiment can be obtained by loading the corresponding SED-ML document into OpenCOR and executing the simulation.

Control of Intracellular Ca^2 + Concentration

The experiment setting to reproduce Figure 2-4 is summarized in the following:

Experiment setting.

Figure [1] shows simulations of Ca^2 +_i decay (in nM). A: Ca^2 +_i decay under inhibition of Ca^2 + pumps. B: Ca^2 +_i decay in control conditions.

Simulated Ca^2 +_i decay (in nM).

Figure [2] shows simulations of Ca^2 +_i rise and decay following a 200 ms voltage pulse from a holding potential of -50 mV to pulse potentials of 0 mV (A), 10 mV (B), and -10 mV (C).

Simulated Ca^2 +_i rise and decay.

Figure [3] shows simulations of Ca^2 +_i rise and decay following a 200 ms voltage pulse from a holding potential of -50 mV to pulse potentials of -20 mV (A) and 20 mV (B).

Simulated Ca^2 +_i rise and decay.

The experiment setting to reproduce primary Figure 5A and 5B are the same as the one used in primary Figure 2B and Figure 3A, respectively.

Figure [4] shows simulations of Ca^2 + fluxes through various Ca^2 + control mechanisms. Plot A shows Ca^2 + flux through Na⁺ ⁄ Ca^2 + exchangers and Ca^2 + pumps during Ca^2 +_i decay for a holding potential of -80 mV followed by a 750 ms voltage pulse to 0 mV, while plot B shows Ca^2 + flux through Ca^2 + channels and Ca^2 + extraction mechanisms during Ca^2 + rise and decay in response to a 200 ms voltage pulse to 0 mV from a holding potential of -50 mV.

Simulated Ca^2 + fluxes through various Ca^2 +_i control mechanisms.